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Preparation and characterization of lipid nanoparticles (LNPs). a Schematic of the preparation of LNPs encapsulating TFEB or S211A mRNA. b Particle size distribution of LNPs. c Cytotoxicity of LNP was determined by CCK-8 assay. d Encapsulation Efficiency of LNP. e Detection of organ distribution of LNP by in vivo luciferase imaging. Mice were injected with 10 μg of luciferase-mRNA encapsulated in LNP via tail vein, followed by an intraperitoneal injection of luciferin 6 h later. Luminescence signals were detected using a small animal in vivo imaging system. f Luminescence signals 6 h, 12 h, 24 h, and 48 h after LNP-luciferase tail-vein injection. g Serum ALT, AST, ALP, and creatinine were measured 24 h after tail-vein injection of 50 μg LNP-luciferase. h In vitro hemolysis assay to assess LNP biocompatibility. Data were expressed as mean ± SEM

Journal: Molecular Biomedicine

Article Title: Lipid nanoparticle-mediated transcription factor EB mRNA delivery for the treatment of metabolic dysfunction-associated steatotic liver disease

doi: 10.1186/s43556-026-00615-0

Figure Lengend Snippet: Preparation and characterization of lipid nanoparticles (LNPs). a Schematic of the preparation of LNPs encapsulating TFEB or S211A mRNA. b Particle size distribution of LNPs. c Cytotoxicity of LNP was determined by CCK-8 assay. d Encapsulation Efficiency of LNP. e Detection of organ distribution of LNP by in vivo luciferase imaging. Mice were injected with 10 μg of luciferase-mRNA encapsulated in LNP via tail vein, followed by an intraperitoneal injection of luciferin 6 h later. Luminescence signals were detected using a small animal in vivo imaging system. f Luminescence signals 6 h, 12 h, 24 h, and 48 h after LNP-luciferase tail-vein injection. g Serum ALT, AST, ALP, and creatinine were measured 24 h after tail-vein injection of 50 μg LNP-luciferase. h In vitro hemolysis assay to assess LNP biocompatibility. Data were expressed as mean ± SEM

Article Snippet: Bioluminescence signals were detected using an in vivo small-animal imaging system (IVIS Spectrum, Revvity).

Techniques: CCK-8 Assay, Encapsulation, In Vivo, Luciferase, Imaging, Injection, In Vivo Imaging, In Vitro, Hemolysis Assay